CDS complement (join(1. .98;62063. .62078)) /gene="1" /product="gp1" /function="hypothetical protein" /locus tag="fizzles_1" /note=Original Glimmer call @bp 98 has strength 5.73 /note=SSC: 98-62063 CP: no SCS: glimmer ST: SS BLAST-Start: GAP: 328 bp gap LO: no RBS: Kibler 6, Karlin Medium, 0.905, -7.539442206964485, no F: hypothetical protein SIF-BLAST: SIF-HHPRED: SIF-Syn: The Phagesdb Function Frequency had no data available, while the Blastp had evidence of hypothetical protein for phages of the same cluster. NKF was select due to lack of consistency in suggested proteins CDS 427 - 1509 /gene="2" /product="gp2" /function="hypothetical protein" /locus tag="fizzles_2" /note=Original Glimmer call @bp 1207 has strength 7.02 /note=SSC: 427-1509 CP: no SCS: glimmer-cs ST: NA BLAST-Start: GAP: 328 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.789, -5.69021729366323, no F: hypothetical protein SIF-BLAST: SIF-HHPRED: U1 small nuclear ribonucleoprotein 70; spliceosme, assemply, pre-B complex, U1; 3.3A {Saccharomyces cerevisiae S288c},,,5ZWN_Q,30.8333,33.7 SIF-Syn: There is a multitude of reasons why the function is still unknown. /note=The PECAAN starterator does not match up with the phagesdb starterator. /note=There is a 23% chance that this is a tape measure protein and a 23% chance that this is a hnh endonuclease. CDS complement (629 - 946) /gene="3" /product="gp3" /function="hypothetical protein" /locus tag="fizzles_3" /note=Original Glimmer call @bp 946 has strength 16.03; Genemark calls start at 946 /note=SSC: 946-629 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein AUG13_06710 [Chloroflexi bacterium 13_1_20CM_2_59_7]],,NCBI, q50:s428 52.381% 1.31452 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.875, -2.9063687850157054, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein AUG13_06710 [Chloroflexi bacterium 13_1_20CM_2_59_7]],,OLE56890,2.74579,1.31452 SIF-HHPRED: Cold shock domain-containing protein E1; CELL-FREE PROTEIN SYNTHESIS, BETA-BARREL, TRANSLATIONAL; NMR {Homo sapiens},,,2YTX_A,36.1905,71.8 SIF-Syn: This gene is not well conserved in EG phages. Previous and subsequent genes are also not well conserved. /note=May be related to withstanding or recovering from damage or stress. Similar to a cold shock protein in Homo sapiens on HHpred. Similar to rtcb-like protein on Phagesdb Function Frequency. CDS complement (943 - 1233) /gene="4" /product="gp4" /function="hypothetical protein" /locus tag="fizzles_4" /note=Original Glimmer call @bp 1233 has strength 9.52; Genemark calls start at 1338 /note=SSC: 1233-943 CP: yes SCS: both-gl ST: SS BLAST-Start: GAP: 101 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.731, -5.509176084620483, no F: hypothetical protein SIF-BLAST: SIF-HHPRED: DUF4451 ; Domain of unknown function (DUF4451),,,PF14616.6,32.2917,45.7 SIF-Syn: Gene is not well conserved in cluster EG. Previous and subsequent genes are also not well conserved closely related phages. /note=Minor tail protein function frequency was 40%, domain of unknown function probability was 45.7%, blast pulled up hypothetical protein. HHPred first hit was domain of unknown function, 47%. HHPred second hit was DNA-binding cysteine-rich domain of male-specific lethal 2 and related proteins 35% and the third hit was PR domain zinc finger protein 31%. CDS complement (1335 - 1493) /gene="5" /product="gp5" /function="hypothetical protein" /locus tag="fizzles_5" /note=Genemark calls start at 1493 /note=SSC: 1493-1335 CP: yes SCS: genemark ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_2 [Microbacterium phage Hyperion]],,NCBI, q9:s11 78.8462% 1.98279E-11 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.969, -4.741344217713455, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_2 [Microbacterium phage Hyperion]],,AWN03520,60.3774,1.98279E-11 SIF-HHPRED: SIF-Syn: This gene doesn`t seem to appear on any other of the closely related phages. /note=No data available in function frequency percentage. Blast gave hypothetical protein as an option. Top hit on HHPred was Thioredoxin as a possible description with probability of 98.5. CDS complement (1490 - 1765) /gene="6" /product="gp6" /function="hypothetical protein" /locus tag="fizzles_6" /note=Original Glimmer call @bp 1765 has strength 6.6; Genemark calls start at 1747 /note=SSC: 1765-1490 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_ONEIAGILLIAN_4 [Microbacterium phage OneinaGillian]],,NCBI, q7:s1 62.6374% 2.93247E-4 GAP: -22 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.048, -4.847583656344026, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ONEIAGILLIAN_4 [Microbacterium phage OneinaGillian]],,AYB70114,34.4828,2.93247E-4 SIF-HHPRED: Zn_ribbon_recom ; Recombinase zinc beta ribbon domain,,,PF13408.6,12.0879,73.7 SIF-Syn: The information did not provided did not show a definitive answer for this protein. /note=Hpred has a 59.15% for transcription factors ll B, 57.95% Transcription initiation factor but phamerator states it could be a histidine nucleotide binding protein. CDS complement (1744 - 1908) /gene="7" /product="gp7" /function="hypothetical protein" /locus tag="fizzles_7" /note=Original Glimmer call @bp 1908 has strength 11.05; Genemark calls start at 1896 /note=SSC: 1908-1744 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein PBI_HENDRIX_39 [Microbacterium phage Hendrix]],,NCBI, q2:s7 72.2222% 0.859913 GAP: -13 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.393, -3.8362837638757656, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HENDRIX_39 [Microbacterium phage Hendrix]],,AWN07710,35.0877,0.859913 SIF-HHPRED: DUF1062 ; Protein of unknown function (DUF1062),,,PF06353.12,14.8148,70.2 SIF-Syn: gene is not well conserved within cluster EG. Previous and subsequent genes are unknown. /note=The evidence is Hendrix, Anakin and many more with having multiple that are similar and have an unknown function. The gene has an unknown function because in PECAAN it shows a 50% frequency and the subclusters are not closely related. In the website`s HHpred (prophage) and Blast (hypothetical protein) shows a different kind of protein. CDS complement (1896 - 2111) /gene="8" /product="gp8" /function="hypothetical protein" /locus tag="fizzles_8" /note=Original Glimmer call @bp 2111 has strength 6.08; Genemark calls start at 2111 /note=SSC: 2111-1896 CP: yes SCS: both ST: SS BLAST-Start: [MARTX multifunctional-autoprocessing repeats-in-toxin holotoxin RtxA [Photorhabdus australis] ],,NCBI, q21:s2702 63.3803% 4.7266 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.953, -3.6514785093170086, yes F: hypothetical protein SIF-BLAST: ,,[MARTX multifunctional-autoprocessing repeats-in-toxin holotoxin RtxA [Photorhabdus australis] ],,WP_065823720,0.757576,4.7266 SIF-HHPRED: DUF29 ; Domain of unknown function DUF29,,,PF01724.16,16.9014,77.3 SIF-Syn: gene is not well conserved within cluster EG. Previous gene function is unknown, subsequent gene function appears to be a histadine triad nucleotide binding protein. /note=NCBI did not find a close enough match to another gene from any of the known phages in the same class. Phamerator also found no matches to any other phages and classified this gene as an orpham. HHPred also found no know matches to any other genes other than hypothetical protein. CDS complement (2108 - 2470) /gene="9" /product="gp9" /function="histidine triad nucleotide binding protein" /locus tag="fizzles_9" /note=Original Glimmer call @bp 2470 has strength 6.61; Genemark calls start at 2470 /note=SSC: 2470-2108 CP: no SCS: both ST: NA BLAST-Start: [histidine triad nucleotide binding protein [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 1.16395E-46 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.781, -3.0425830684175623, yes F: histidine triad nucleotide binding protein SIF-BLAST: ,,[histidine triad nucleotide binding protein [Microbacterium phage Hyperion]],,AWN03524,71.0744,1.16395E-46 SIF-HHPRED: HIT-like protein hinT; histidine triad nucleotide binding protein; HET: 5GP, EDO; 1.45A {Escherichia coli},,,3N1S_J,94.1667,99.9 SIF-Syn: Gene is not well conserved within EG, but it has an abundance of members in its pham report. They are often reported at histidine triad nucleotide binding proteins. Previous and subsequent gene functions are unknown. /note=On HHpred, fizzles gene 9 hits with histidine coding comparisons mentioning also "automated matches". /note=On BLAST, fizzles gene 9 first two matches are 100% histidine coding. Also mentions family protein and domain containing proteins. CDS complement (2467 - 2634) /gene="10" /product="gp10" /function="hypothetical protein" /locus tag="fizzles_10" /note=Original Glimmer call @bp 2634 has strength 6.21; Genemark calls start at 2634 /note=SSC: 2634-2467 CP: yes SCS: both ST: SS BLAST-Start: GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.711, -3.170856472917156, yes F: hypothetical protein SIF-BLAST: SIF-HHPRED: SIF-Syn: Does not seem to be similar to genes of the closest related phages. /note=PhagesDB contained no starterator data. Blastp did not find any significant similarities. CDS complement (2631 - 3173) /gene="11" /product="gp11" /function="membrane protein" /locus tag="fizzles_11" /note=Original Glimmer call @bp 3173 has strength 15.95; Genemark calls start at 3173 /note=SSC: 3173-2631 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Squash]],,NCBI, q1:s1 98.3333% 4.84393E-65 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.729, -3.0713502170045657, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Squash]],,AWN04629,50.4167,4.84393E-65 SIF-HHPRED: NMN_transporter ; Nicotinamide mononucleotide transporter,,,PF04973.12,43.8889,98.3 SIF-Syn: The positioning of the gene and the similarities to other phages justify that this is a membrane protein. HHpred and NCBI Blast have provided proof that these findings are correct. /note=On the NCBI Blast tool on PECAAN, the identity percentage (46.7%) is much lower than the percentage I am getting on the NCBI website (62.9%). I have refreshed the programs several times, and it continues to produce the same data. CDS complement (3170 - 3691) /gene="12" /product="gp12" /function="ssDNA binding protein" /locus tag="fizzles_12" /note=Original Glimmer call @bp 3691 has strength 10.68; Genemark calls start at 3691 /note=SSC: 3691-3170 CP: yes SCS: both ST: SS BLAST-Start: [ssDNA binding protein [Microbacterium phage Squash]],,NCBI, q5:s5 97.1098% 1.22473E-87 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.465, -5.79598068500287, no F: ssDNA binding protein SIF-BLAST: ,,[ssDNA binding protein [Microbacterium phage Squash]],,AWN04631,83.237,1.22473E-87 SIF-HHPRED: SIF-Syn: Mashley and Hyperion have a ssDNA binding protein that follows a membrane protein. /note=The NCBI Blastp and PECAAN showed around a 72 and 75% match with three phages that had the function of ssDNA binding protein. CDS complement (3688 - 3933) /gene="13" /product="gp13" /function="ribonucleotide reductase" /locus tag="fizzles_13" /note=Original Glimmer call @bp 3933 has strength 19.76; Genemark calls start at 3933 /note=SSC: 3933-3688 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein BGN98_13760 [Microbacterium sp. 69-7]],,NCBI, q9:s14 87.6543% 2.21292 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.92, -5.114865311845495, no F: ribonucleotide reductase SIF-BLAST: ,,[hypothetical protein BGN98_13760 [Microbacterium sp. 69-7]],,OJU44446,41.4141,2.21292 SIF-HHPRED: SIF-Syn: I believe that is its function is ribonucleotide reductase because of the graph given it being so similar to the other phages. /note=The evidence is L5, XianYue, and Babyray with all three being similar to fizzles and having ribonucleotide reductase as their functions. The frequency % for ribonucleotide reductase is 67% for subcluster A2 and 33% for subcluster A3. CDS complement (3930 - 4178) /gene="14" /product="gp14" /function="hypothetical protein" /locus tag="fizzles_14" /note=Original Glimmer call @bp 4178 has strength 12.39; Genemark calls start at 4178 /note=SSC: 4178-3930 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_14 [Microbacterium phage Squash]],,NCBI, q1:s1 100.0% 1.13129E-27 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.164, -4.314571574115886, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_14 [Microbacterium phage Squash]],,AWN04633,75.6098,1.13129E-27 SIF-HHPRED: SIF-Syn: /note=This gene has an unknown function, because it is does not have a strong probability on HHpred or Blastp indicating it`s similarity to one particular protein. CDS complement (4175 - 4531) /gene="15" /product="gp15" /function="helix-turn-helix DNA binding domain" /locus tag="fizzles_15" /note=Original Glimmer call @bp 4531 has strength 6.93; Genemark calls start at 4531 /note=SSC: 4531-4175 CP: yes SCS: both ST: SS BLAST-Start: [helix-turn-helix DNA binding protein [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 9.97552E-79 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.731, -5.220380545373514, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding protein [Microbacterium phage Hyperion] ],,AWN03529,99.1525,9.97552E-79 SIF-HHPRED: HTH_41 ; Helix-turn-helix domain,,,PF14502.6,29.661,60.3 SIF-Syn: When compared to other phages, the placement and positioning of the gene shows similarity between it and helix-turn-helices on the other phages. This means that it makes sense for this to be a helix-turn helix as well. /note=The results for the gene on Pecaan and HHpred did not match in chronological order, but based on their percent identity it can be predicted that the function is helix-turn-helix DNA binding domain. NCBI shows 96.61% and HH pred shows 98.53% probability. While it was clear that both had helix-turn-helix domains, the genes themselves had several differences. The top result on Pecaan was found to be the 117th result of HHpred. CDS complement (4524 - 4739) /gene="16" /product="gp16" /function="helix-turn-helix DNA binding domain" /locus tag="fizzles_16" /note=Original Glimmer call @bp 4739 has strength 6.56; Genemark calls start at 4682 /note=SSC: 4739-4524 CP: yes SCS: both-gl ST: SS BLAST-Start: [helix-turn-helix DNA binding protein [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 1.93087E-36 GAP: 61 bp gap LO: no RBS: Kibler 6, Karlin Medium, 0.817, -7.134752732491032, no F: helix-turn-helix DNA binding domain SIF-BLAST: ,,[helix-turn-helix DNA binding protein [Microbacterium phage Hyperion]],,AWN03530,92.9577,1.93087E-36 SIF-HHPRED: Regulatory protein cox; helix-turn-helix, DNA binding, VIRAL PROTEIN; 2.401A {Enterobacteria phage P2},,,4LHF_A,85.9155,98.8 SIF-Syn: Based on the phamerator, rudy Ralph shows a similar protein function at the sequence number 16 with similar with size. They are both at the transcribe forward on the sequence as well. /note=Based on the NCB blast, the function indicates a helix- turn- helix protein at a pecernt identity of 40.91%. HHpred does not indicate a helix turn helix at the beginning but rather at NR 8 with a with propability of 98. 95 %. CDS 4801 - 4956 /gene="17" /product="gp17" /function="ribbon-helix-helix DNA binding domain" /locus tag="fizzles_17" /note=Original Glimmer call @bp 4801 has strength 10.67; Genemark calls start at 4843 /note=SSC: 4801-4956 CP: yes SCS: both-gl ST: SS BLAST-Start: [DNA binding domain protein [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 2.83813E-12 GAP: 61 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.891, -6.130624125819145, no F: ribbon-helix-helix DNA binding domain SIF-BLAST: ,,[DNA binding domain protein [Microbacterium phage Hyperion] ],,AWN03531,76.4706,2.83813E-12 SIF-HHPRED: NikA; DIMER, BACTERIAL CONJUGATION, RELAXASE, DNA; NMR {Plasmid R64},,,2BA3_A,92.1569,99.3 SIF-Syn: Phages Squash, Hyperion, and Mashley all have this as their first forward transcribed protein. The closest match on Blastp is a DNA binding domain protein. There are similar proteins that are with ribbon-helix-helix and/or DNA binding domain in their name on HHpred. /note=Based on NCBI Blast there is a percent identity of 58.82% that the function is a DNA binding protein. HH Pred states that CDS 4953 - 5597 /gene="18" /product="gp18" /function="hypothetical protein" /locus tag="fizzles_18" /note=Original Glimmer call @bp 4953 has strength 18.74; Genemark calls start at 4953 /note=SSC: 4953-5597 CP: yes SCS: both ST: SS BLAST-Start: [ribbon-helix-helix DNA binding domain protein [Microbacterium phage Squash]],,NCBI, q5:s34 97.6636% 1.53301E-83 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.029, -2.583959800616441, yes F: hypothetical protein SIF-BLAST: ,,[ribbon-helix-helix DNA binding domain protein [Microbacterium phage Squash]],,AWN04637,67.9842,1.53301E-83 SIF-HHPRED: HTH_40 ; Helix-turn-helix domain,,,PF14493.6,17.757,93.6 SIF-Syn: There are several reasons for why the function is still unknown /note=The gene is closely related to helix-turn-helix dna-binding domain (52%). The next one that it is most similar to is the capsid decoration protein. CDS 5599 - 5763 /gene="19" /product="gp19" /function="hypothetical protein" /locus tag="fizzles_19" /note=Original Glimmer call @bp 5599 has strength 9.56; Genemark calls start at 5599 /note=SSC: 5599-5763 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_ONEIAGILLIAN_17 [Microbacterium phage OneinaGillian]],,NCBI, q5:s3 92.5926% 4.53431E-7 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.965, -4.731360067354143, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ONEIAGILLIAN_17 [Microbacterium phage OneinaGillian]],,AYB70127,69.2308,4.53431E-7 SIF-HHPRED: HlyD_D4 ; Long alpha hairpin domain of cation efflux system protein, CusB,,,PF16572.5,53.7037,80.6 SIF-Syn: There are a variety of reasons why the function is still unknown /note=Frequency of a tape measure protein at 100% CDS 5763 - 5939 /gene="20" /product="gp20" /function="hypothetical protein" /locus tag="fizzles_20" /note=Original Glimmer call @bp 5763 has strength 12.19; Genemark calls start at 5763 /note=SSC: 5763-5939 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_16 [Microbacterium phage Hyperion]],,NCBI, q6:s7 91.3793% 4.69867E-22 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.389, -6.526515810972826, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_16 [Microbacterium phage Hyperion]],,AWN03533,79.661,4.69867E-22 SIF-HHPRED: zf-Sec23_Sec24 ; Sec23/Sec24 zinc finger,,,PF04810.15,20.6897,73.1 SIF-Syn: Genes within this pham in closely related phages (Mashley, Hyperion, and Squash) are hypothetical proteins as well. /note=Could be a hypothetical protein as shown on blast, according to the HHPred hit list, the gene could function in some sort of oxidation-reduction purpose, with 79.26% probability. CDS 5939 - 6301 /gene="21" /product="gp21" /function="tail assembly chaperone" /locus tag="fizzles_21" /note=Original Glimmer call @bp 5939 has strength 11.33; Genemark calls start at 5939 /note=SSC: 5939-6301 CP: yes SCS: both ST: SS BLAST-Start: [ribbon-helix-helix DNA binding domain protein [Microbacterium phage Hyperion] ],,NCBI, q4:s3 97.5% 2.51874E-46 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.969, -4.741344217713455, no F: tail assembly chaperone SIF-BLAST: ,,[ribbon-helix-helix DNA binding domain protein [Microbacterium phage Hyperion] ],,AWN03534,79.0323,2.51874E-46 SIF-HHPRED: NikA; DIMER, BACTERIAL CONJUGATION, RELAXASE, DNA; NMR {Plasmid R64},,,2BA3_A,37.5,94.0 SIF-Syn: Due to the placement of this gene on other closely related phages. /note=Could also be ribbon-helix-helix DNA binding domain protein due to blast or DNA binding protein, as seen on Squash. 87% frequency for tail assembly chaperone CDS 6291 - 7814 /gene="22" /product="gp22" /function="terminase" /locus tag="fizzles_22" /note=Original Glimmer call @bp 6291 has strength 13.16; Genemark calls start at 6303 /note=SSC: 6291-7814 CP: yes SCS: both-gl ST: SS BLAST-Start: [terminase [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 0.0 GAP: -11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.596, -3.410574749511644, no F: terminase SIF-BLAST: ,,[terminase [Microbacterium phage Hyperion] ],,AWN03535,88.2704,0.0 SIF-HHPRED: Large subunit terminase; large terminase, VIRAL PROTEIN; 2.2A {Deep-sea thermophilic phage D6E},,,5OE8_C,84.2209,100.0 SIF-Syn: Squash, Hyperion, and Mashley have terminases in the same pham and in similar spots in their genomes. /note=100% probability to be a terminase according to HHPred and 78.6% identity to terminase on BLASTp. HHPred claims 100% probability to be the large subunit of a terminase, could possibly be there is a small subunit in the genome. CDS 7811 - 8155 /gene="23" /product="gp23" /function="membrane protein" /locus tag="fizzles_23" /note=Original Glimmer call @bp 7811 has strength 13.07; Genemark calls start at 7811 /note=SSC: 7811-8155 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_19 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 1.40827E-54 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.668, -3.1994059460302813, yes F: membrane protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_19 [Microbacterium phage Hyperion] ],,AWN03536,89.4737,1.40827E-54 SIF-HHPRED: SIF-Syn: The protein has the potential to be a membrane protein due to its positioning and data findings. /note=NCBI 100% confidence of hypothetical protein. Phagesdb membrane protein 100% confidence. Membrane protein, NCBI has membrane protein as a possibility. The phagesdb has a high percentage of membrane protein. Phamerator positioning has a strong indication of the findings in. CDS 8440 - 10269 /gene="24" /product="gp24" /function="portal protein" /locus tag="fizzles_24" /note=Original Glimmer call @bp 8440 has strength 16.75; Genemark calls start at 8440 /note=SSC: 8440-10269 CP: yes SCS: both ST: SS BLAST-Start: [portal protein [Microbacterium phage Hyperion] ],,NCBI, q1:s1 97.3727% 0.0 GAP: 284 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.707, -3.196631460098011, no F: portal protein SIF-BLAST: ,,[portal protein [Microbacterium phage Hyperion] ],,AWN03538,86.5132,0.0 SIF-HHPRED: Phage_portal ; Phage portal protein,,,PF04860.12,59.2775,99.9 SIF-Syn: Mashley, Squash, and Hyperion have portal proteins in a similar location in their genomes. /note=HHPred showed 98.9% probability to be a portal protein. BLASTp showed 79.53% identity to a portal protein. CDS 13469 - 13630 /gene="25" /product="gp25" /function="major capsid protein" /locus tag="fizzles_25" /note=Original Glimmer call @bp 13469 has strength 5.86; Genemark calls start at 13469 /note=SSC: 13469-13630 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_23 [Microbacterium phage Hyperion]],,NCBI, q1:s1 94.3396% 7.61147E-12 GAP: 3199 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.547, -3.5316035464369326, yes F: major capsid protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_23 [Microbacterium phage Hyperion]],,AWN03540,56.6667,7.61147E-12 SIF-HHPRED: SIF-Syn: The gene location and size is similar to those of Mashely and Hyperion`s major capsid protein, giving fizzle`s gene 26 a high probability of being a major capsid protein /note=It is believed to be a hypothetical protein from the Blastp results. Sea-phages functional assignment shows no data on hypothetical proteins. Phagesdb function frequency shows that it could be a head-to-tail connector protein. Gene seems to match a major capsid gene from phages Mashely and Hyperion. CDS 13768 - 15600 /gene="26" /product="gp26" /function="major capsid protein" /locus tag="fizzles_26" /note=Original Glimmer call @bp 13768 has strength 15.92; Genemark calls start at 13768 /note=SSC: 13768-15600 CP: no SCS: both ST: SS BLAST-Start: [major capsid protein [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 0.0 GAP: 137 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.442961286954254, yes F: major capsid protein SIF-BLAST: ,,[major capsid protein [Microbacterium phage Hyperion]],,AWN03541,85.1974,0.0 SIF-HHPRED: SIF-Syn: Compared to other similar bacteriophages (Mashley, Hyperion, and Squash), the percent identity (77.5%) provided proof that this is a major capsid protein. After running the material through NCBI and HHpred, I confirmed this information. /note=HHpred showed a 54% probability of it being a major capsid protein. CDS 15665 - 16411 /gene="27" /product="gp27" /function="hypothetical protein" /locus tag="fizzles_27" /note=Original Glimmer call @bp 15665 has strength 13.09; Genemark calls start at 15665 /note=SSC: 15665-16411 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_25 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 98.3871% 3.99649E-115 GAP: 64 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.274, -2.2821867859826788, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_25 [Microbacterium phage Hyperion] ],,AWN03542,77.459,3.99649E-115 SIF-HHPRED: SIF-Syn: NCBI blastp showed a 98% match with 3 other phages labeled “hypothetical protein.” Cannot find any other related function on HHpred. CDS 16422 - 16925 /gene="28" /product="gp28" /function="hypothetical protein" /locus tag="fizzles_28" /note=Original Glimmer call @bp 16422 has strength 15.22; Genemark calls start at 16422 /note=SSC: 16422-16925 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_25 [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 1.32956E-42 GAP: 10 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.274, -1.9310779259753799, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_25 [Microbacterium phage Mashley]],,QED11842,64.6707,1.32956E-42 SIF-HHPRED: SIF-Syn: Table unknown. /note=All of the other phages similar to this one is unknown. CDS 16958 - 17899 /gene="29" /product="gp29" /function="major tail protein" /locus tag="fizzles_29" /note=Original Glimmer call @bp 16958 has strength 12.96; Genemark calls start at 16955 /note=SSC: 16958-17899 CP: yes SCS: both-gl ST: NI BLAST-Start: [major tail protein [Microbacterium phage Hyperion] ],,NCBI, q1:s2 99.6805% 0.0 GAP: 32 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.363, -4.187763896562409, no F: major tail protein SIF-BLAST: ,,[major tail protein [Microbacterium phage Hyperion] ],,AWN03544,87.619,0.0 SIF-HHPRED: SIF-Syn: Blastp shows strong probability that this gene is a major tail protein. /note=HHpred indicates it might be a surface tail protein, but Blastp showed stronger evidence. CDS 18023 - 19351 /gene="30" /product="gp30" /function="hypothetical protein" /locus tag="fizzles_30" /note=Original Glimmer call @bp 18023 has strength 12.1; Genemark calls start at 18023 /note=SSC: 18023-19351 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein ABS60_00295 [Microbacterium sp. SCN 71-17] ],,NCBI, q175:s536 39.819% 2.14131E-31 GAP: 123 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.953, -2.6013996449736907, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein ABS60_00295 [Microbacterium sp. SCN 71-17] ],,ODT43226,13.5236,2.14131E-31 SIF-HHPRED: SIF-Syn: There is not enough similarity in the other phages to make connections. /note=If anything, this could be a major tail protein due to a small amount of similarity in the general positioning of the gene. However, there was no facts to back this up. CDS 19351 - 20256 /gene="31" /product="gp31" /function="hydrolase" /locus tag="fizzles_31" /note=Original Glimmer call @bp 19351 has strength 14.13; Genemark calls start at 19351 /note=SSC: 19351-20256 CP: yes SCS: both ST: NI BLAST-Start: [MULTISPECIES: alpha/beta hydrolase [unclassified Rhodococcus] ],,NCBI, q1:s1 85.0498% 7.46352E-75 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.363, -4.188334187302651, no F: hydrolase SIF-BLAST: ,,[MULTISPECIES: alpha/beta hydrolase [unclassified Rhodococcus] ],,WP_094640257,54.0193,7.46352E-75 SIF-HHPRED: Esterase D; alpha beta hydrolase, catalytic triad; 1.6A {Lactobacillus rhamnosus},,,3DKR_A,80.7309,99.8 SIF-Syn: Mellie shows similarity base on sequence number given. The sequence is smaller on Mellie but they have a similar gene base on size. They are also forward. /note=Base on NCBI blast the protein indicated a hydrolase at a 51.4% identity with a querry cover of 85%. The HHpred did not indicated it was a hydrolase base on the chronological order. The Nr was at 7 with a 99.89 % propability. The start and end codon ended with similar digits according to the Pham 94971. CDS 20286 - 21131 /gene="32" /product="gp32" /function="head-to-tail adaptor" /locus tag="fizzles_32" /note=Original Glimmer call @bp 20286 has strength 12.04; Genemark calls start at 20286 /note=SSC: 20286-21131 CP: yes SCS: both ST: SS BLAST-Start: [head-to-tail adaptor [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 3.05717E-143 GAP: 29 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.187, -2.17469248771465, yes F: head-to-tail adaptor SIF-BLAST: ,,[head-to-tail adaptor [Microbacterium phage Mashley]],,QED11846,80.1418,3.05717E-143 SIF-HHPRED: PORTAL PROTEIN, 15 PROTEIN, HEAD; VIRAL PROTEIN, VIRAL INFECTION, TAILED; 7.2A {BACILLUS PHAGE SPP1},,,5A21_C,26.3345,94.9 SIF-Syn: In Phamerator the protein matches up with head-to-tail adaptor proteins on the other Cluster EG phages. The first couple hits on Blastp say head-to-tail adaptor. Several hits on HHpred say head-to-tail connector. /note=Based on NCBI Blast there is a percent identity of 69.15% of the function head-to-tail-adaptor. HH pred indicates a probability of 91.89% that it is also a head-to-tail adaptor. CDS 21131 - 21658 /gene="33" /product="gp33" /function="hypothetical protein" /locus tag="fizzles_33" /note=Original Glimmer call @bp 21131 has strength 14.04; Genemark calls start at 21131 /note=SSC: 21131-21658 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_31 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 1.35186E-93 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.41, -5.893810279580483, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_31 [Microbacterium phage Hyperion] ],,AWN03548,86.3636,1.35186E-93 SIF-HHPRED: MerB2; Bacterial Proteins, Cysteine, Lyases, Mercury; HET: GOL, DTV; 1.24A {Bacillus megaterium},,,5C17_A,32.0,27.7 SIF-Syn: There are several reasons for why the function is still unknown. /note=This is most likely a minor capsid protein (100%). CDS 21660 - 22067 /gene="34" /product="gp34" /function="hypothetical protein" /locus tag="fizzles_34" /note=Original Glimmer call @bp 21660 has strength 9.12; Genemark calls start at 21660 /note=SSC: 21660-22067 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_35 [Microbacterium phage Squash]],,NCBI, q1:s1 98.5185% 1.52136E-41 GAP: 1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.293, -4.045036357814993, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_35 [Microbacterium phage Squash]],,AWN04654,73.7226,1.52136E-41 SIF-HHPRED: HK97-gp10_like ; Bacteriophage HK97-gp10, putative tail-component,,,PF04883.12,50.3704,98.8 SIF-Syn: There are several reasons for why the function is unknown. /note=There is a 24% frequency of being a tail assembly chaperone. CDS 22106 - 22564 /gene="35" /product="gp35" /function="tail assembly chaperone" /locus tag="fizzles_35" /note=Original Glimmer call @bp 22106 has strength 14.16; Genemark calls start at 22115 /note=SSC: 22106-22564 CP: no SCS: both-gl ST: SS BLAST-Start: [tail assembly chaperone [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 1.12755E-74 GAP: 38 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.029, -2.442961286954254, yes F: tail assembly chaperone SIF-BLAST: ,,[tail assembly chaperone [Microbacterium phage Hyperion]],,AWN03550,82.2368,1.12755E-74 SIF-HHPRED: SIF-Syn: In phamerator, the gene seems to be conserved within other phages of cluster EG, such as Squash, Hyperion, and Mashley. /note=tail assembly chaperone had a 47% function frequency. blastp also had 100% query coverage of tail assembly chaperone for the similar phages. CDS 22770 - 25841 /gene="36" /product="gp36" /function="tape measure protein" /locus tag="fizzles_36" /note=Original Glimmer call @bp 22770 has strength 12.43; Genemark calls start at 22770 /note=SSC: 22770-25841 CP: yes SCS: both ST: SS BLAST-Start: [tape measure protein [Microbacterium phage Squash]],,NCBI, q1:s1 100.0% 0.0 GAP: 205 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.129, -4.4057176022952405, no F: tape measure protein SIF-BLAST: ,,[tape measure protein [Microbacterium phage Squash]],,AWN04657,81.8526,0.0 SIF-HHPRED: Nucleoporin_FG2 ; Nucleoporin FG repeated region,,,PF15967.5,20.5279,85.0 SIF-Syn: longest gene in genome. located after capsid, portal, and capsid maturation structural protein. located before tail protein genes. there is only one. /note=hhpred does not have a tapemeasure match, but in comparison to other phages and synteny, this gene should be the tapemeasure. CDS 25850 - 27190 /gene="37" /product="gp37" /function="minor tail protein" /locus tag="fizzles_37" /note=Original Glimmer call @bp 25850 has strength 14.43; Genemark calls start at 25850 /note=SSC: 25850-27190 CP: no SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 0.0 GAP: 8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.129, -4.4057176022952405, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Mashley]],,QED11852,83.1839,0.0 SIF-HHPRED: SIF-Syn: This gene is conserved within the other phages of this cluster, Hyperion, Squash, and Mashley, all have this gene in similar locations on the genome. /note=In BLAST it query cover of 100% but in PECAAN the highest frequency% was 31. All the hits in PECAAN show other phages in the cluster have this gene as a minor tail protein. CDS 27219 - 30158 /gene="38" /product="gp38" /function="minor tail protein" /locus tag="fizzles_38" /note=Original Glimmer call @bp 27219 has strength 15.49; Genemark calls start at 27219 /note=SSC: 27219-30158 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 0.0 GAP: 28 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.617, -4.213475109731446, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage Mashley]],,QED11853,79.445,0.0 SIF-HHPRED: Leukemia inhibitory factor receptor/Leukemia inhibitory; CYTOKINE CELL SURFACE RECEPTOR COMPLEX; HET: NAG, FUC, MAN; 4.0A {Mus musculus},,,2Q7N_C,47.7017,99.9 SIF-Syn: Minor Tail Protein, NCBI and Phagedb have both come to the same findings. /note=NCBI minor tail protein, 100% confidence. HHpred and Conserved Domain Database to not contain evidence to support this conclusion. CDS 30158 - 31312 /gene="39" /product="gp39" /function="minor tail protein" /locus tag="fizzles_39" /note=Original Glimmer call @bp 30158 has strength 14.34; Genemark calls start at 30158 /note=SSC: 30158-31312 CP: yes SCS: both ST: SS BLAST-Start: [minor tail protein [Microbacterium phage OneinaGillian]],,NCBI, q6:s5 98.4375% 0.0 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.653, -3.2297885750240023, no F: minor tail protein SIF-BLAST: ,,[minor tail protein [Microbacterium phage OneinaGillian]],,AYB70148,83.5079,0.0 SIF-HHPRED: SIF-Syn: NCBI found E-values of 0.0 for minor tail proteins in Hyperion, Squash, OneinaGillian and Mashley. Pharmerator showed gene in a similar location as other phages` minor tail proteins from the same class; Squash and RubyRalph: gene 41, OneinaGillian: gene 38, Mashley: gene 37, and Hyperion: gene 38. HHPred visualization was majority red along with hitlist showing tail proteins. /note=The NCBI had a range of 98% - 95% of major tail protein, the phamerator also has strong indication of this information.HHpred has a variety of indications however tail protein is an option. CDS 31312 - 32058 /gene="40" /product="gp40" /function="minor tail protein" /locus tag="fizzles_40" /note=Original Glimmer call @bp 31312 has strength 11.92; Genemark calls start at 31345 /note=SSC: 31312-32058 CP: no SCS: both-gl ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_39 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 1.57171E-76 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.875, -2.8454123590742793, yes F: minor tail protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_39 [Microbacterium phage Hyperion] ],,AWN03555,66.0079,1.57171E-76 SIF-HHPRED: SIF-Syn: CDS 32069 - 32329 /gene="41" /product="gp41" /function="membrane protein" /locus tag="fizzles_41" /note=Original Glimmer call @bp 32069 has strength 15.45; Genemark calls start at 32069 /note=SSC: 32069-32329 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_SUCHA_19 [Microbacterium phage Sucha]],,NCBI, q1:s1 88.3721% 9.19411E-20 GAP: 10 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.129, -4.387988835334809, no F: membrane protein SIF-BLAST: ,,[hypothetical protein SEA_SUCHA_19 [Microbacterium phage Sucha]],,QCQ57464,67.4699,9.19411E-20 SIF-HHPRED: Class B acid phosphatase (E.C.3.1.3.2); Apha class B Acid Phosphatase/Phosphotransferase; 1.5A {Escherichia coli} SCOP: c.108.1.12,,,2HEG_B,23.2558,51.5 SIF-Syn: Gene 42 seems to match Mashely and Hyperion`s membrane protein in location and size. /note=HHpred give a 52% probability that it could be a RNA-binding protein. Phagesdb functions frequency shows a 64% probability that it could be a minor tail protein. Because of these similarities in location and size of the gene, with Mashely and Hyperion, it contains a high chance of being a membrane protein. CDS 32402 - 32860 /gene="42" /product="gp42" /function="membrane protein" /locus tag="fizzles_42" /note=Original Glimmer call @bp 32402 has strength 13.43; Genemark calls start at 32402 /note=SSC: 32402-32860 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Hyperion] ],,NCBI, q1:s1 99.3421% 5.6549E-54 GAP: 72 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.12, -2.394485424036831, yes F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Hyperion] ],,AWN03557,79.2208,5.6549E-54 SIF-HHPRED: SIF-Syn: Phagesdb blast shows the highest probability bacteriophage (Casend) is an unknown gene. HHpred has an 86% probability of being a zinc binding protein. /note=phagesdb function frequency and phagesdb blast do not match with HHpred`s results. CDS 32850 - 33041 /gene="43" /product="gp43" /function="hypothetical protein" /locus tag="fizzles_43" /note=Original Glimmer call @bp 32850 has strength 10.39; Genemark calls start at 32850 /note=SSC: 32850-33041 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_42 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 6.09711E-19 GAP: -11 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.953, -3.2535385006449706, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_42 [Microbacterium phage Hyperion] ],,AWN03558,78.4615,6.09711E-19 SIF-HHPRED: SIF-Syn: NCBI blastp showed hypothetical protein while PECAAN and HHpred showed unknown function. CDS 33043 - 33789 /gene="44" /product="gp44" /function="membrane protein" /locus tag="fizzles_44" /note=Original Glimmer call @bp 33043 has strength 12.81; Genemark calls start at 33043 /note=SSC: 33043-33789 CP: yes SCS: both ST: SS BLAST-Start: [membrane protein [Microbacterium phage Squash]],,NCBI, q1:s1 98.7903% 1.241E-76 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.366, -3.9718914447963414, no F: membrane protein SIF-BLAST: ,,[membrane protein [Microbacterium phage Squash]],,AWN04665,73.0612,1.241E-76 SIF-HHPRED: SIF-Syn: Transmembrane prediction also shows it is membrane protein. There is a frequency % of membrane protein of 16% for subcluster AS2, 11% for subcluster EB, and 10% for subcluster DC1. /note=Many of the similar phages have membrane protein functions. CDS 33875 - 34684 /gene="45" /product="gp45" /function="hypothetical protein" /locus tag="fizzles_45" /note=Original Glimmer call @bp 33875 has strength 16.9; Genemark calls start at 33869 /note=SSC: 33875-34684 CP: no SCS: both-gl ST: NI BLAST-Start: [lysin A [Microbacterium phage Hyperion]],,NCBI, q1:s3 65.0558% 6.47345E-66 GAP: 85 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.731, -5.299065738275371, no F: hypothetical protein SIF-BLAST: ,,[lysin A [Microbacterium phage Hyperion]],,AWN03560,48.7179,6.47345E-66 SIF-HHPRED: SIF-Syn: This gene is unknown because the databases were not lining up similarly, though there is somewhat a strong percentage that it is a lysin A protein. /note=High chance that this gene is a lysin A since membrane protein comes before it and Blastp shows a somewhat strong percentage that it is a lysin A protein. CDS complement (34759 - 35256) /gene="46" /product="gp46" /function="hypothetical protein" /locus tag="fizzles_46" /note=Original Glimmer call @bp 35256 has strength 6.11; Genemark calls start at 35151 /note=SSC: 35256-34759 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_46 [Microbacterium phage Hyperion]],,NCBI, q1:s1 99.3939% 1.44009E-61 GAP: -32 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.346, -3.8730906109286094, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_46 [Microbacterium phage Hyperion]],,AWN03561,77.0588,1.44009E-61 SIF-HHPRED: DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation.,,,cd00268,26.0606,67.1 SIF-Syn: The genes before and after this gene are very similar to that of Hyperion. The format is the same for these two, as well as Mashley. /note=HHpred gives a 93.96% chance that this gene is a DEAD-box helicase. However, this requires different genes to preceed than those in fizzles. All reports conflict, with different genes being probable. CDS complement (35225 - 35527) /gene="47" /product="gp47" /function="hypothetical protein" /locus tag="fizzles_47" /note=Original Glimmer call @bp 35527 has strength 5.24; Genemark calls start at 35527 /note=SSC: 35527-35225 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_46 [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 1.88241E-44 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.346, -6.492722648163624, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_46 [Microbacterium phage Mashley]],,QED11863,90.0,1.88241E-44 SIF-HHPRED: MPN_DUF2466; Mov34/MPN/PAD-1 family. Mov34 DUF2466 (also known as DNA repair protein RadC) domain of unknown function contains the signature JAB1/MPN/Mov34 metalloenzyme (JAMM) motif, EXnHS/THX7SXXD, which is involved in zinc ion coordination and provides the active site for isopeptidase activity.,,,cd08071,17.0,69.7 SIF-Syn: Base on the phages db blast there are unknown function base on the phages given. OneinaGillian is fairly similar to it base on the phamerator. The sequence lenegth is similar and they are both backwards. /note=Base on the NCBI blast there is a function of desaturase at 57.69 percent, but on HHpred there is a high probability that the function is not known at 82.17 % probability. desaturase does not appear on HHpred. CDS complement (35524 - 36189) /gene="48" /product="gp48" /function="chaperonin, DnaJ-like" /locus tag="fizzles_48" /note=Original Glimmer call @bp 36189 has strength 13.02; Genemark calls start at 36189 /note=SSC: 36189-35524 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_47 [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 1.0598E-88 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.791, -5.032659008396601, no F: chaperonin, DnaJ-like SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_47 [Microbacterium phage Mashley]],,QED11864,75.9091,1.0598E-88 SIF-HHPRED: Mitochondrial import inner membrane translocase; DnaJ-fold, CHAPERONE, PROTEIN TRANSPORT; HET: FLC; 2.0A {Saccharomyces cerevisiae},,,2GUZ_J,30.3167,98.5 SIF-Syn: On Phamerator, rudyRalph indicates a closer identity of gene length compare to other genes below. /note=There`s a high possibility that gene 48 is a chaperonin accoding to HH pred and NCBI blast. HH pred indicates a 98.28% probability and NCBI blast shows 52.06% identity to chaperonin. CDS complement (36186 - 36770) /gene="49" /product="gp49" /function="MazG-like nucleotide pyrophosphohydrolase" /locus tag="fizzles_49" /note=Original Glimmer call @bp 36770 has strength 13.89; Genemark calls start at 36770 /note=SSC: 36770-36186 CP: yes SCS: both ST: SS BLAST-Start: [MULTISPECIES: haloacid dehalogenase [Bacillus] ],,NCBI, q10:s14 64.9485% 1.16664E-32 GAP: -1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.048, -4.637473309998914, no F: MazG-like nucleotide pyrophosphohydrolase SIF-BLAST: ,,[MULTISPECIES: haloacid dehalogenase [Bacillus] ],,WP_042980443,56.338,1.16664E-32 SIF-HHPRED: MAZG-LIKE NUCLEOSIDE TRIPHOSPHATE PYROPHOSPHOHYDROLASE (E.C.3.6.1.19); HYDROLASE, DIMERIC DUTPASE; HET: GOL, SO4; 1.7A {DEINOCOCCUS RADIODURANS},,,2YF4_F,65.4639,100.0 SIF-Syn: Frequency is at 48% similarities /note=HH pred indicates a 99.94% probability of MazG- like nucleotide pryphosphydrolase. NCBI blast did indicate a similarity. CDS complement (36770 - 37699) /gene="50" /product="gp50" /function="hypothetical protein" /locus tag="fizzles_50" /note=Original Glimmer call @bp 37699 has strength 16.97; Genemark calls start at 37699 /note=SSC: 37699-36770 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein [Leifsonia aquatica]],,NCBI, q1:s1 98.0583% 1.5155E-103 GAP: 24 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.583959800616441, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Leifsonia aquatica]],,WP_025157260,67.5325,1.5155E-103 SIF-HHPRED: DNA TOPOISOMERASE VI (E.C.5.99.1.3) A; TOPOISOMERASE VI, DNA BINDING PROTEIN; 2.0A {Methanocaldococcus jannaschii} SCOP: e.12.1.1,,,1D3Y_A,83.8188,99.8 SIF-Syn: There are several reasons for why the function is unknown. /note=This gene is an orpham, so there is no data CDS complement (37724 - 39934) /gene="51" /product="gp51" /function="hypothetical protein" /locus tag="fizzles_51" /note=Original Glimmer call @bp 39934 has strength 12.38; Genemark calls start at 39934 /note=SSC: 39934-37724 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_53 [Microbacterium phage Squash]],,NCBI, q1:s1 99.7283% 0.0 GAP: -11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_53 [Microbacterium phage Squash]],,AWN04672,79.8331,0.0 SIF-HHPRED: DUF2800 ; Protein of unknown function (DUF2800),,,PF10926.8,51.2228,99.0 SIF-Syn: All the other phages in the cluster have a similar gene that is also of unknown function. This seems enough evidence to mark it as NKF. /note=Major capside protein has a frequency of 13% as well as tape measure protein at 13%; however, tape measure protein is already identified as gene 37. Hypothetical protein also came up with 99% probability. CDS complement (39924 - 40166) /gene="52" /product="gp52" /function="minor tail protein" /locus tag="fizzles_52" /note=Original Glimmer call @bp 40166 has strength 7.25; Genemark calls start at 40166 /note=SSC: 40166-39924 CP: yes SCS: both ST: SS BLAST-Start: GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.471, -3.750998330397795, yes F: minor tail protein SIF-BLAST: SIF-HHPRED: DUF589; alpha-beta, RpR3, NESG, Structural Genomics; NMR {Rhodopseudomonas palustris} SCOP: b.122.1.8,,,2GBS_A,43.75,33.7 SIF-Syn: Minor tail protein, this gene is not on the other closely related phages, it seems to be an insertion. /note=Frequency 100% for minor tail protein. Tape measure protein was also given for the blast; however, this protein was already identified. CDS complement (40166 - 41170) /gene="53" /product="gp53" /function="major tail protein" /locus tag="fizzles_53" /note=Original Glimmer call @bp 41170 has strength 14.84; Genemark calls start at 41170 /note=SSC: 41170-40166 CP: no SCS: both ST: NI BLAST-Start: [DNA recombinase [Microbacterium phage Mashley]],,NCBI, q1:s1 99.4012% 1.61534E-107 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.583959800616441, yes F: major tail protein SIF-BLAST: ,,[DNA recombinase [Microbacterium phage Mashley]],,QED11867,68.9349,1.61534E-107 SIF-HHPRED: SIF-Syn: Phamerator placement is similar to the major tail protein. /note=NCBI major tail protein is 59%. Phagedb is majority tape measure protein. HHpred is 99.1% homologous-pairing domain of Rad52 recombinase and 99.9% on DNA repair protein. CDS complement (41167 - 41709) /gene="54" /product="gp54" /function="HNH endonuclease" /locus tag="fizzles_54" /note=Original Glimmer call @bp 41709 has strength 9.7; Genemark calls start at 41709 /note=SSC: 41709-41167 CP: yes SCS: both ST: SS BLAST-Start: [HNH endonuclease [Microbacterium phage OneinaGillian]],,NCBI, q1:s1 99.4444% 1.05782E-68 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.743, -4.647348953121449, yes F: HNH endonuclease SIF-BLAST: ,,[HNH endonuclease [Microbacterium phage OneinaGillian]],,AYB70170,71.3483,1.05782E-68 SIF-HHPRED: HNH_3 ; HNH endonuclease,,,PF13392.6,24.4444,98.7 SIF-Syn: This gene in blast has a precent identify at 60% as HNH endonuclease but in HHpred it contains another protein function /note=Changed to HNH endonuclease to reflect the evidence presented in the google doc, phagesdb, blast, and HHpred. According to the HHPred table, probability was 98.7 for HNH endonuclease. CDS complement (41706 - 42284) /gene="55" /product="gp55" /function="RuvC-like resolvase" /locus tag="fizzles_55" /note=Original Glimmer call @bp 42284 has strength 13.29; Genemark calls start at 42284 /note=SSC: 42284-41706 CP: yes SCS: both ST: SS BLAST-Start: [RuvC-like resolvase [Microbacterium phage Hyperion] ],,NCBI, q5:s5 97.9167% 2.46357E-69 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.162, -4.909000519077716, no F: RuvC-like resolvase SIF-BLAST: ,,[RuvC-like resolvase [Microbacterium phage Hyperion] ],,AWN03568,72.1053,2.46357E-69 SIF-HHPRED: HOLLIDAY JUNCTION RESOLVASE (E.C.3.1.22.4) (RUVC); SITE-SPECIFIC RECOMBINASE; 2.5A {Escherichia coli} SCOP: c.55.3.6,,,1HJR_C,86.9792,99.9 SIF-Syn: NCBI found E-values close to 0.0 for RuvC-like resolvase in Hyperion, Squash, and OneinaGillian. Pharmerator showed gene in a similar location as other phages from the same class; Squash: gene 56, OneinaGillian: gene 61, Mashley: gene 52, and Hyperion: gene 53, RubyRalph: gene 59. HHPred visualization was majority red along with hitlist showing resolvase. CDS complement (42281 - 42472) /gene="56" /product="gp56" /function="hypothetical protein" /locus tag="fizzles_56" /note=Original Glimmer call @bp 42472 has strength 10.14; Genemark calls start at 42472 /note=SSC: 42472-42281 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_54 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 87.3016% 5.75112E-19 GAP: 147 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.932, -2.6453814847322845, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_54 [Microbacterium phage Hyperion] ],,AWN03569,67.2131,5.75112E-19 SIF-HHPRED: SIF-Syn: tRNA complement (42504 - 42577) /gene="57" /product="tRNA-Arg(CGT)" /locus tag="FIZZLES_57" /note=tRNA-Arg(CGT) CDS complement (42620 - 43354) /gene="58" /product="gp58" /function="ssDNA binding protein" /locus tag="fizzles_58" /note=Original Glimmer call @bp 43354 has strength 10.59; Genemark calls start at 43354 /note=SSC: 43354-42620 CP: no SCS: both ST: SS BLAST-Start: [single-stranded DNA-binding protein [Microbacterium sp. AR7-10] ],,NCBI, q1:s1 59.4262% 2.09698E-73 GAP: 1 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.875, -2.827683592113848, yes F: ssDNA binding protein SIF-BLAST: ,,[single-stranded DNA-binding protein [Microbacterium sp. AR7-10] ],,WP_071641239,74.3902,2.09698E-73 SIF-HHPRED: Crystal Structure of Single-stranded DNA-binding; Single-stranded DNA-binding protein, Streptomyces Coelicolor; 2.141A {Streptomyces coelicolor},,,3EIV_D,52.8689,99.9 SIF-Syn: All date received for this gene shows that it is a ssDNA binding protein. Blastp showed over an 80% similarities to those of a ssDNA binding protein. HHpred shows a near 100% probability of it being a ssDNA binding protein. /note=Blastp shows a high percentage of being a ssDNA binding protein. Gene is similar in size and location in comparison to Mashley and Hyperion`s ssDNA binding protein. HHpred showed a near 100% probability of it being a ssDNA CDS complement (43356 - 43535) /gene="59" /product="gp59" /function="hypothetical protein" /locus tag="fizzles_59" /note=Original Glimmer call @bp 43535 has strength 23.01; Genemark calls start at 43535 /note=SSC: 43535-43356 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_ARIADNE_73 [Microbacterium phage Ariadne] ],,NCBI, q1:s3 98.3051% 2.20559E-16 GAP: 15 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.778, -3.0301037898918786, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_ARIADNE_73 [Microbacterium phage Ariadne] ],,QGJ89476,68.8525,2.20559E-16 SIF-HHPRED: DNA (cytosine-5)-methyltransferase 3B (E.C.2.1.1.37); DNMT3B PWWP DOMAIN, HISTONE BINDING; HET: 96E, SO4; 1.7A {Homo sapiens},,,5NRR_B,62.7119,88.8 SIF-Syn: This gene has an unknown function; therefore, it is classified as NKF. This is supported by similar bacteriophages with similar hypothetical proteins. /note=HHpred has a 72% probability of it being a gene with an unknown function. NCBL blast shows a 52% probability of it being an unknown function. CDS complement (43551 - 43751) /gene="60" /product="gp60" /function="hypothetical protein" /locus tag="fizzles_60" /note=Original Glimmer call @bp 43751 has strength 11.55; Genemark calls start at 43751 /note=SSC: 43751-43551 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_59 [Microbacterium phage Hyperion]],,NCBI, q1:s1 95.4545% 4.41454E-6 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.135, -4.376977241479107, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_59 [Microbacterium phage Hyperion]],,AWN03574,48.2353,4.41454E-6 SIF-HHPRED: SIF-Syn: NCBI blastp and HHpred both result in hypothetical protein. /note=No data available. CDS complement (43748 - 43981) /gene="61" /product="gp61" /function="hypothetical protein" /locus tag="fizzles_61" /note=Original Glimmer call @bp 43981 has strength 14.15; Genemark calls start at 43981 /note=SSC: 43981-43748 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_63 [Microbacterium phage Squash]],,NCBI, q5:s4 93.5065% 6.01464E-33 GAP: 80 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.029, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_63 [Microbacterium phage Squash]],,AWN04682,83.3333,6.01464E-33 SIF-HHPRED: SIF-Syn: /note=This appears to be an unknown function because the phages most similar to this one all have unknown functions. CDS complement (44062 - 44175) /gene="62" /product="gp62" /function="hypothetical protein" /locus tag="fizzles_62" /note=Original Glimmer call @bp 44175 has strength 11.84; Genemark calls start at 44172 /note=SSC: 44175-44062 CP: yes SCS: both-gl ST: NA BLAST-Start: [hypothetical protein PBI_HYPERION_61 [Microbacterium phage Hyperion] ],,NCBI, q5:s6 89.1892% 1.82872E-9 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.086, -4.416689425358505, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_61 [Microbacterium phage Hyperion] ],,AWN03576,73.6842,1.82872E-9 SIF-HHPRED: SIF-Syn: This gene has an unknown function, because it is does not have a strong probability on HHpred or Blastp indicating it`s similarity to one particular protein. CDS complement (44172 - 44564) /gene="63" /product="gp63" /function="hypothetical protein" /locus tag="fizzles_63" /note=Original Glimmer call @bp 44564 has strength 10.11; Genemark calls start at 44564 /note=SSC: 44564-44172 CP: yes SCS: both ST: SS BLAST-Start: [3-phosphoshikimate 1-carboxyvinyltransferase [Lactobacillus capillatus] ],,NCBI, q21:s332 50.7692% 0.525639 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.891, -4.905314844093283, no F: hypothetical protein SIF-BLAST: ,,[3-phosphoshikimate 1-carboxyvinyltransferase [Lactobacillus capillatus] ],,WP_057745391,8.92449,0.525639 SIF-HHPRED: Zinc finger CCCH-type with G; transcription regulation, structural genomics consortium; HET: UNX; 2.65A {Homo sapiens},,,4II1_D,43.8462,30.9 SIF-Syn: There is no similarity between this gene and others. /note=The highest percentages anywhere were under 60%. CDS complement (44561 - 44836) /gene="64" /product="gp64" /function="hypothetical protein" /locus tag="fizzles_64" /note=Original Glimmer call @bp 44836 has strength 6.44; Genemark calls start at 44836 /note=SSC: 44836-44561 CP: no SCS: both ST: NI BLAST-Start: [bacterial extracellular solute-binding protein family 3 [Veillonella sp. CAG:933]],,NCBI, q2:s163 34.0659% 8.54481 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.676, -3.243963255891538, yes F: hypothetical protein SIF-BLAST: ,,[bacterial extracellular solute-binding protein family 3 [Veillonella sp. CAG:933]],,CCX53868,7.35294,8.54481 SIF-HHPRED: UPF0223 protein BH2638; pfam, UPF0223, 10286b, Structural Genomics; 1.6A {Bacillus halodurans C-125} SCOP: a.276.1.1,,,2OY9_A,36.2637,70.6 SIF-Syn: I predict that it is closely related to phage TG1 on phages DB. It must be known that a Starterator has not been determine with the archives. /note=Base on the function, it is consider an NKF because NCBI blast states that it is a transporter protein at 51.61% and the HHpred mentions it is not a transporter protein at any percentage. The closest protein will be a Homoprotocatechuate 2,3-dioxygenase at 76.65%. The phages DB does not an Starterator to analyze as well. CDS complement (44836 - 45120) /gene="65" /product="gp65" /function="hypothetical protein" /locus tag="fizzles_65" /note=Original Glimmer call @bp 45120 has strength 14.58; Genemark calls start at 45120 /note=SSC: 45120-44836 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_66 [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 7.224E-47 GAP: 0 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.065, -4.601664605840989, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_66 [Microbacterium phage Hyperion]],,AWN03581,88.2979,7.224E-47 SIF-HHPRED: ArsA_HSP20 ; HSP20-like domain found in ArsA,,,PF17886.1,20.2128,61.1 SIF-Syn: Phamerator shows a close relationship between Fizzles and NIke. Their relationship is base on on size and they`re both on the backward strand on the strand. /note=Based on HH-pred, there is a 60.37 % probability that gene 64 has an unknown function. NCBI blast estimates a 78.72% hypothetical function (UKF). The gene has an a UNF fucntion. CDS complement (45121 - 45639) /gene="66" /product="gp66" /function="hypothetical protein" /locus tag="fizzles_66" /note=Original Glimmer call @bp 45639 has strength 9.65; Genemark calls start at 45639 /note=SSC: 45639-45121 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Frigoribacterium sp. PhB118] ],,NCBI, q110:s13 24.4186% 9.20961E-14 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.352, -3.922401531015186, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Frigoribacterium sp. PhB118] ],,WP_123570403,49.2958,9.20961E-14 SIF-HHPRED: cytidine and deoxycytidylate deaminase; RNA Editing, RNA BINDING PROTEIN; 1.6A {Agrobacterium tumefaciens} SCOP: c.97.1.2,,,2A8N_B,51.7442,78.8 SIF-Syn: There is a multitude of reasons for why the function is still unknown. /note=Could possibly be hnh endonuclease because there is a frequency of 57%. Could possibly be deaminase. There`s a 76.66%. CDS complement (45636 - 45752) /gene="67" /product="gp67" /function="hypothetical protein" /locus tag="fizzles_67" /note=Original Glimmer call @bp 45752 has strength 9.47; Genemark calls start at 45755 /note=SSC: 45752-45636 CP: yes SCS: both-gl ST: SS BLAST-Start: [FtsX-like permease family protein [Bacillus sp. FJAT-42376] ],,NCBI, q6:s300 73.6842% 5.7722 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.366, -3.9718914447963414, no F: hypothetical protein SIF-BLAST: ,,[FtsX-like permease family protein [Bacillus sp. FJAT-42376] ],,WP_123919251,2.44845,5.7722 SIF-HHPRED: EcCorA_ZntB-like; Escherichia coli CorA-Salmonella typhimurium ZntB_like family. A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.,,,cd12821,50.0,70.7 SIF-Syn: There is a multitude of reasons for why the function is still unknown. /note=There is a 100% frequency for membrane protein CDS complement (45749 - 45994) /gene="68" /product="gp68" /function="hypothetical protein" /locus tag="fizzles_68" /note=Original Glimmer call @bp 45994 has strength 5.26; Genemark calls start at 45994 /note=SSC: 45994-45749 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein EON53_09945 [Actinomycetales bacterium]],,NCBI, q9:s4 87.6543% 0.440724 GAP: -14 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.794070146961553, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein EON53_09945 [Actinomycetales bacterium]],,RYY45858,16.0448,0.440724 SIF-HHPRED: DUF4969 ; Domain of unknown function (DUF4969),,,PF16339.5,29.6296,90.2 SIF-Syn: NKF /note=No data available CDS complement (45981 - 47954) /gene="69" /product="gp69" /function="DNA primase/helicase" /locus tag="fizzles_69" /note=Original Glimmer call @bp 47954 has strength 10.55; Genemark calls start at 47954 /note=SSC: 47954-45981 CP: yes SCS: both ST: SS BLAST-Start: [DNA primase [Microbacterium phage Squash]],,NCBI, q1:s1 100.0% 0.0 GAP: 178 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.563, -5.5915060314559355, no F: DNA primase/helicase SIF-BLAST: ,,[DNA primase [Microbacterium phage Squash]],,AWN04690,91.6667,0.0 SIF-HHPRED: DNA primase (E.C.2.7.7.-); Zinc Ribbon, TOPRIM, RNA POLYMERASE; 2.0A {Aquifex aeolicus},,,2AU3_A,34.7032,99.7 SIF-Syn: Due to the placement of the gene, a DNA primase/helicase would make sense as compared to Squash. /note=DNA recombinase was 27% on the function frequency. In squash it was DNA primase, with only 44% query cover. DNA Primase/helicase was 100% probability on HHPred. CDS complement (48133 - 49488) /gene="70" /product="gp70" /function="major tail protein" /locus tag="fizzles_70" /note=Original Glimmer call @bp 49488 has strength 19.13; Genemark calls start at 49488 /note=SSC: 49488-48133 CP: yes SCS: both ST: NI BLAST-Start: [hypothetical protein SEA_ONEIAGILLIAN_74 [Microbacterium phage OneinaGillian]],,NCBI, q1:s50 83.3703% 2.24522E-165 GAP: 101 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.932, -3.1725816037952645, no F: major tail protein SIF-BLAST: ,,[hypothetical protein SEA_ONEIAGILLIAN_74 [Microbacterium phage OneinaGillian]],,AYB70184,45.1659,2.24522E-165 SIF-HHPRED: SIF-Syn: The protein shows characteristics of being major tail protein based of its placement on phamerator. /note=NCBI is 49% major tail protein. HHpred is 71.28% gram pos anchor. CDS complement (49590 - 49673) /gene="71" /product="gp71" /function="hypothetical protein" /locus tag="fizzles_71" /note=Original Glimmer call @bp 49901 has strength 13.69; Genemark calls start at 49901 /note=SSC: 49673-49590 CP: yes SCS: both-cs ST: SS BLAST-Start: GAP: 217 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.875, -2.7653702713535186, yes F: hypothetical protein SIF-BLAST: SIF-HHPRED: ARIA_NM_RDCfit_1; RRM domain RBD domain.; NMR {Mus musculus},,,2F3J_A,92.5926,82.9 SIF-Syn: It does not have a known function and the subcluster is not similar to the cluster /note=The genome position does have a variation of 49901 to 49590 (Reverse) within Phagedb (070) and within PECAAN (070) 49901 to 49901. NCBI shows a large support for minor tail protein. HHpred 95.27% on transcriptional repressor COG. CDS complement (49891 - 50088) /gene="72" /product="gp72" /function="hypothetical protein" /locus tag="fizzles_72" /note=Original Glimmer call @bp 50088 has strength 12.69; Genemark calls start at 50088 /note=SSC: 50088-49891 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_72 [Microbacterium phage Hyperion] ],,NCBI, q1:s29 90.7692% 3.54378E-13 GAP: 80 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.521, -4.812051156217375, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_72 [Microbacterium phage Hyperion] ],,AWN03587,49.4382,3.54378E-13 SIF-HHPRED: SIF-Syn: NCBI found only three matches for hypothetical proteins in Hyperion, Squash, and OneinaGillian. Pharmerator showed gene in a similar location as other phages from the same class; Squash: gene 75, OneinaGillian: gene 76, Mashley: gene 74, and Hyperion: gene 72, RubyRalph: gene 72. HHPred visualization was not very conclusive and was considered not applicable. CDS complement (50169 - 50513) /gene="73" /product="gp73" /function="hypothetical protein" /locus tag="fizzles_73" /note=Original Glimmer call @bp 50513 has strength 17.83; Genemark calls start at 50513 /note=SSC: 50513-50169 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_77 [Microbacterium phage Squash]],,NCBI, q1:s1 94.7368% 3.18314E-40 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.442, -4.197870532213559, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_77 [Microbacterium phage Squash]],,AWN04695,71.6814,3.18314E-40 SIF-HHPRED: SIF-Syn: CDS complement (50506 - 50820) /gene="74" /product="gp74" /function="hypothetical protein" /locus tag="fizzles_74" /note=Original Glimmer call @bp 50820 has strength 19.3; Genemark calls start at 50820 /note=SSC: 50820-50506 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_74 [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 1.1004E-40 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.029, -2.970161406017234, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_74 [Microbacterium phage Hyperion]],,AWN03589,78.6408,1.1004E-40 SIF-HHPRED: DUF1590 ; Protein of unknown function (DUF1590),,,PF07629.11,12.5,56.9 SIF-Syn: The blastp, HHpred show data that gives this gene a high probability of being a protein of unknown function. Shares similar size and gene location with that of Hyperion and Mashley, though the gene function is unknown. /note=PhagesDB shows gene as an orpham; does not provide starterator data. HHpred shows 96% probability of being a protein of unknown function. Shows a frequency of 67% CDS complement (50813 - 51163) /gene="75" /product="gp75" /function="hypothetical protein" /locus tag="fizzles_75" /note=Original Glimmer call @bp 51163 has strength 11.17; Genemark calls start at 51163 /note=SSC: 51163-50813 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Enterobacter roggenkampii] ],,NCBI, q4:s3 46.5517% 6.36244E-7 GAP: -8 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.891, -4.887586077132851, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Enterobacter roggenkampii] ],,WP_039266277,55.0,6.36244E-7 SIF-HHPRED: DUF551 ; Protein of unknown function (DUF551),,,PF04448.12,47.4138,95.9 SIF-Syn: This is a common protein with no known function. It will be classified as NKF because it`s function is unknown. /note=This was difficult because Phagesdb was relating this gene to a minor tail protein; however, HHpred and NCBI have only bacteriophages with proteins of unknown function to compare it to; HHpred showed a 96% probability of it being an unknown function. However, the relation on NCBI has a percent identity of only 36.6%. CDS complement (51156 - 51416) /gene="76" /product="gp76" /function="hypothetical protein" /locus tag="fizzles_76" /note=Original Glimmer call @bp 51416 has strength 17.84; Genemark calls start at 51416 /note=SSC: 51416-51156 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_75 [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 1.95521E-28 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.012, -2.5588120788329394, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_75 [Microbacterium phage Hyperion]],,AWN03590,74.4186,1.95521E-28 SIF-HHPRED: SIF-Syn: NCBI Blastp revealed "hypothetical protein." HHpred showed no other information. /note=No data available when refreshed. CDS complement (51413 - 51679) /gene="77" /product="gp77" /function="RecA-like DNA Recombinase" /locus tag="fizzles_77" /note=Original Glimmer call @bp 51679 has strength 5.91; Genemark calls start at 51679 /note=SSC: 51679-51413 CP: no SCS: both ST: SS BLAST-Start: [MULTISPECIES: HIT domain-containing protein [Pseudomonas] ],,NCBI, q20:s45 53.4091% 9.66349 GAP: 8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.467, -6.238906642477926, no F: RecA-like DNA Recombinase SIF-BLAST: ,,[MULTISPECIES: HIT domain-containing protein [Pseudomonas] ],,WP_054898003,18.4397,9.66349 SIF-HHPRED: SIF-Syn: The phagesdb function frequency % for DNA recombinase is 67%. /note=The most similar phage has a DNA recombinase function. CDS complement (51688 - 52017) /gene="78" /product="gp78" /function="hypothetical protein" /locus tag="fizzles_78" /note=Original Glimmer call @bp 52017 has strength 17.42; Genemark calls start at 52017 /note=SSC: 52017-51688 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_78 [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 3.69885E-66 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.97, -4.738619112134128, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_78 [Microbacterium phage Hyperion]],,AWN03593,97.2477,3.69885E-66 SIF-HHPRED: SIF-Syn: This gene has an unknown function, because it is does not have a strong probability on HHpred or Blastp indicating it`s similarity to one particular protein. CDS complement (52017 - 52286) /gene="79" /product="gp79" /function="nucleotide pyrophosphohydrolase" /locus tag="fizzles_79" /note=Original Glimmer call @bp 52286 has strength 16.06; Genemark calls start at 52286 /note=SSC: 52286-52017 CP: yes SCS: both ST: SS BLAST-Start: [nucleotide pyrophosphohydrolase [Microbacterium phage Hyperion] ],,NCBI, q4:s7 95.5056% 5.10555E-40 GAP: 2 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.029, -3.3503726477288405, yes F: nucleotide pyrophosphohydrolase SIF-BLAST: ,,[nucleotide pyrophosphohydrolase [Microbacterium phage Hyperion] ],,AWN03591,81.3187,5.10555E-40 SIF-HHPRED: MazG-related protein; TM0360, Left-handed superhelix fold, Structural; 2.0A {Thermotoga maritima},,,2YXH_B,56.1798,74.7 SIF-Syn: There is great similarity with hyperion gene 76 and mashley gene 78 /note=All reports match well, as well as the synteny with hyperion. CDS complement (52289 - 52615) /gene="80" /product="gp80" /function="hypothetical protein" /locus tag="fizzles_80" /note=Original Glimmer call @bp 52615 has strength 16.04; Genemark calls start at 52615 /note=SSC: 52615-52289 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_80 [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 8.75668E-54 GAP: 11 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.52, -3.50848394673489, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_80 [Microbacterium phage Hyperion]],,AWN03595,85.1852,8.75668E-54 SIF-HHPRED: DUF982 ; Protein of unknown function (DUF982),,,PF06169.12,36.1111,72.8 SIF-Syn: Based on the phamerator, the phage squash has a similar gene size and number on the sequence. They also share the hypothetical as known as no function compare to the other phages on the phages DB blast /note=Based on the NCBI data, there is hypothetical function which means that there is no function. All of the given options are hypothetical at a range of 75% to 57%. The software HHpred shows hypothetical at a 43.88 %. On the Nr 5. Phages db blast showed phages that were all hypothetical. CDS complement (52627 - 52917) /gene="81" /product="gp81" /function="hypothetical protein" /locus tag="fizzles_81" /note=Original Glimmer call @bp 52917 has strength 13.28; Genemark calls start at 52917 /note=SSC: 52917-52627 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein SEA_ONEIAGILLIAN_85 [Microbacterium phage OneinaGillian]],,NCBI, q67:s31 31.25% 2.37414E-5 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.114, -4.497860114175419, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ONEIAGILLIAN_85 [Microbacterium phage OneinaGillian]],,AYB70195,43.3333,2.37414E-5 SIF-HHPRED: GINLFY PROTEIN; SAM, TRANSCRIPTION, OLIGOMERISATION; HET: GOL, PG4; 2.25A {GINKGO BILOBA},,,4UDE_B,52.0833,69.0 SIF-Syn: Phamerator has no matches with proteins of known function. NCBI has only one hit which is a hypothetical protein. HHpred did not have a very definitive answer for what the function is either. /note=NCBI blast indicated a hypothetical function (NKF) at 73.33% identity. HH pred did not show any hypothetical function. CDS complement (52914 - 53420) /gene="82" /product="gp82" /function="hypothetical protein" /locus tag="fizzles_82" /note=Original Glimmer call @bp 53420 has strength 17.71; Genemark calls start at 53420 /note=SSC: 53420-52914 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_83 [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 1.5713E-64 GAP: 2 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -3.3503726477288405, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_83 [Microbacterium phage Mashley]],,QED11899,72.619,1.5713E-64 SIF-HHPRED: Abhydrolase_1 ; alpha/beta hydrolase fold,,,PF00561.20,26.1905,48.4 SIF-Syn: There are several reasons for why the function is still unknown. /note=Could be minor tail protein with a frequency of 57% or DNA polymerase I with a frequency of 24% CDS complement (53423 - 53875) /gene="83" /product="gp83" /function="hypothetical protein" /locus tag="fizzles_83" /note=Original Glimmer call @bp 53875 has strength 16.11; Genemark calls start at 53875 /note=SSC: 53875-53423 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_87 [Microbacterium phage Squash]],,NCBI, q1:s1 100.0% 1.3457E-55 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.065, -4.601664605840989, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_87 [Microbacterium phage Squash]],,AWN04705,82.5503,1.3457E-55 SIF-HHPRED: Swi5-dependent recombination DNA repair protein; recombination activator; HET: GOL; 2.2A {Schizosaccharomyces pombe},,,3VIQ_A,34.0,69.1 SIF-Syn: There are several reasons for why the function is still unknown. /note=No data is available for function frequency. CDS complement (53872 - 54192) /gene="84" /product="gp84" /function="hypothetical protein" /locus tag="fizzles_84" /note=Original Glimmer call @bp 54192 has strength 18.73; Genemark calls start at 54192 /note=SSC: 54192-53872 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_85 [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 6.11247E-51 GAP: 9 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.12, -2.3335289980954053, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_85 [Microbacterium phage Hyperion]],,AWN03600,86.7924,6.11247E-51 SIF-HHPRED: Cytoskeleton assembly control protein SLA1; Sla1, SHD1, NPFx(1,2)D, endocytosis, PROTEIN; NMR {Saccharomyces cerevisiae},,,2HBP_A,33.0189,80.7 SIF-Syn: NKF /note=There is a 50% chance that this is a minor tail protein and a 50% chance that this dna polymerase i CDS complement (54202 - 54555) /gene="85" /product="gp85" /function="hypothetical protein" /locus tag="fizzles_85" /note=Original Glimmer call @bp 54555 has strength 11.64; Genemark calls start at 54555 /note=SSC: 54555-54202 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_86 [Microbacterium phage Hyperion]],,NCBI, q5:s3 95.7265% 1.78704E-40 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.442, -3.75071250087134, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_86 [Microbacterium phage Hyperion]],,AWN03601,76.5217,1.78704E-40 SIF-HHPRED: SIF-Syn: NKF /note=Capsid maturation protease came up with 59% frequency. Coronavirus nucleocapsid protein came up on HHPred as the top hit with 87 probability. CDS complement (54552 - 55139) /gene="86" /product="gp86" /function="hypothetical protein" /locus tag="fizzles_86" /note=Original Glimmer call @bp 55139 has strength 14.96; Genemark calls start at 55139 /note=SSC: 55139-54552 CP: yes SCS: both ST: NA BLAST-Start: [hypothetical protein PBI_HYPERION_87 [Microbacterium phage Hyperion]],,NCBI, q1:s14 100.0% 1.75985E-97 GAP: 36 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.442961286954254, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_87 [Microbacterium phage Hyperion]],,AWN03602,75.9615,1.75985E-97 SIF-HHPRED: SIF-Syn: The importation does not provide a definite function of the protein. /note=NCBI is 100% on the protein being hypothetical. /note=HHpred has similarities to fungal proteins, (IVB, IVD, IVE) and polo like kinase. CDS complement (55176 - 55427) /gene="87" /product="gp87" /function="hypothetical protein" /locus tag="fizzles_87" /note=Original Glimmer call @bp 55427 has strength 16.1; Genemark calls start at 55427 /note=SSC: 55427-55176 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_88 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 93.9759% 4.95229E-28 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.021, -4.902637746059556, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_88 [Microbacterium phage Hyperion] ],,AWN03603,73.1707,4.95229E-28 SIF-HHPRED: SIF-Syn: This gene shows two different function such as protein kinase and hypothetical protein according to HHpred therefore the full function is unknown. /note=NCBI shows indications that 56% major tail protein. Phamerator map shows variation however strong evidence for the tail protein. CDS complement (55424 - 55828) /gene="88" /product="gp88" /function="hypothetical protein" /locus tag="fizzles_88" /note=Original Glimmer call @bp 55828 has strength 11.65; Genemark calls start at 55828 /note=SSC: 55828-55424 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein [Microbacterium sp. ZOR0019]],,NCBI, q1:s3 100.0% 3.09801E-20 GAP: 204 bp gap LO: no RBS: Kibler 6, Karlin Medium, 3.166, -2.6835611257758942, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein [Microbacterium sp. ZOR0019]],,WP_047523115,55.814,3.09801E-20 SIF-HHPRED: DUF2321 ; Uncharacterized protein conserved in bacteria (DUF2321),,,PF10083.9,44.7761,95.8 SIF-Syn: NCBI found only matches for hypothetical proteins in Hyperion, Squash, and others. Pharmerator showed gene in a similar location as Hyperion: gene 72. HHPred visualization was found approximately half red and presented a prediction of zinc ribbons, but was considered not applicable. CDS complement (56033 - 56470) /gene="89" /product="gp89" /function="hypothetical protein" /locus tag="fizzles_89" /note=Original Glimmer call @bp 56470 has strength 14.23; Genemark calls start at 56470 /note=SSC: 56470-56033 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_89 [Microbacterium phage Hyperion]],,NCBI, q1:s1 100.0% 6.96211E-70 GAP: -1 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.875, -3.1164791313608173, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_89 [Microbacterium phage Hyperion]],,AWN03604,87.234,6.96211E-70 SIF-HHPRED: SIF-Syn: CDS complement (56470 - 57096) /gene="90" /product="gp90" /function="hypothetical protein" /locus tag="fizzles_90" /note=Original Glimmer call @bp 57096 has strength 10.88; Genemark calls start at 57096 /note=SSC: 57096-56470 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein E6R04_08315 [Spirochaetes bacterium]],,NCBI, q88:s94 55.7692% 2.84766E-4 GAP: 99 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.627, -4.113180079823027, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein E6R04_08315 [Spirochaetes bacterium]],,TXH09345,25.8065,2.84766E-4 SIF-HHPRED: Gmx_para_CXXCG ; Protein of unknown function (Gmx_para_CXXCG),,,PF09535.10,18.75,70.8 SIF-Syn: Comparing it to similar genes from similar phages, the function of the gene is still not known. HHpred results in a 72% probability of an unknown protein. /note=Frequency is 67% for a portal protein. Data from Blastp shows that it could be an unknown protein. HHpred data has a 72% probability of being an unknown protein. CDS complement (57196 - 57426) /gene="91" /product="gp91" /function="hypothetical protein" /locus tag="fizzles_91" /note=Original Glimmer call @bp 57426 has strength 13.18; Genemark calls start at 57426 /note=SSC: 57426-57196 CP: no SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_95 [Microbacterium phage Squash]],,NCBI, q1:s1 100.0% 3.36588E-24 GAP: -8 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.557, -3.5719506502182776, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_95 [Microbacterium phage Squash]],,AWN04713,76.0,3.36588E-24 SIF-HHPRED: Heterocyst differentiation control protein (E.C.3.4.21.-); single helix, loop packing around; 2.049A {Nostoc},,,4LH9_A,19.7368,81.6 SIF-Syn: phagesdb frequency shows an equal percentage for five possible functions. Bacteriophage (squash) is the closest related phage and it shows an unknown function. /note=The gene was compared to a similar gene of bacteriophages in the same cluster. However, Phagesdb believes that this protein has a function. NCBI has provided the bacteriophage Squash with a percent identity of almost 70% to justify this classification. CDS complement (57419 - 57613) /gene="92" /product="gp92" /function="hypothetical protein" /locus tag="fizzles_92" /note=Original Glimmer call @bp 57613 has strength 8.71; Genemark calls start at 57613 /note=SSC: 57613-57419 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ONEIAGILLIAN_97 [Microbacterium phage OneinaGillian]],,NCBI, q1:s1 89.0625% 1.94087E-9 GAP: 24 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 3.029, -2.5052746077145835, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ONEIAGILLIAN_97 [Microbacterium phage OneinaGillian]],,AYB70207,63.4921,1.94087E-9 SIF-HHPRED: SIF-Syn: NCBI Blastp showed hypothetical protein. HHpred provided no further discovery. /note=No data available when refreshed. CDS complement (57638 - 57979) /gene="93" /product="gp93" /function="hypothetical protein" /locus tag="fizzles_93" /note=Original Glimmer call @bp 57979 has strength 11.92; Genemark calls start at 57979 /note=SSC: 57979-57638 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_ONEIAGILLIAN_98 [Microbacterium phage OneinaGillian]],,NCBI, q1:s1 92.9204% 5.71993E-64 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.52, -3.649482460397077, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_ONEIAGILLIAN_98 [Microbacterium phage OneinaGillian]],,AYB70208,96.1905,5.71993E-64 SIF-HHPRED: SIF-Syn: /note=All similar phages have unknown functions. CDS complement (57976 - 58113) /gene="94" /product="gp94" /function="hypothetical protein" /locus tag="fizzles_94" /note=Original Glimmer call @bp 58113 has strength 16.72; Genemark calls start at 58113 /note=SSC: 58113-57976 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_95 [Microbacterium phage Mashley]],,NCBI, q1:s1 100.0% 1.03194E-7 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.02, -4.696275508110696, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_95 [Microbacterium phage Mashley]],,QED11911,75.5556,1.03194E-7 SIF-HHPRED: SIF-Syn: This gene has an unknown function, because it is does not have a strong probability on HHpred or Blastp indicating it`s similarity to one particular protein. CDS complement (58110 - 58346) /gene="95" /product="gp95" /function="hypothetical protein" /locus tag="fizzles_95" /note=Original Glimmer call @bp 58346 has strength 18.82; Genemark calls start at 58346 /note=SSC: 58346-58110 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_99 [Microbacterium phage Squash]],,NCBI, q1:s1 94.8718% 1.10432E-25 GAP: -4 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.098, -4.532304881730868, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_99 [Microbacterium phage Squash]],,AWN04717,72.8395,1.10432E-25 SIF-HHPRED: SIF-Syn: The format is very similar to Squash gene 99. /note=All reports conflict, but it is similar to a hypothetical protein from Squash CDS complement (58343 - 58798) /gene="96" /product="gp96" /function="hypothetical protein" /locus tag="fizzles_96" /note=Original Glimmer call @bp 58798 has strength 13.42; Genemark calls start at 58798 /note=SSC: 58798-58343 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_100 [Microbacterium phage Squash]],,NCBI, q1:s9 100.0% 1.05968E-72 GAP: 37 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 1.553, -5.53214920859642, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_100 [Microbacterium phage Squash]],,AWN04718,79.1139,1.05968E-72 SIF-HHPRED: CcmH_N; N-terminal domain of cytochrome c-type biogenesis protein CcmH and related proteins.,,,cd16378,21.8543,83.0 SIF-Syn: Based on the gene 96 and the phamerator. The closest phage with similar function is Mashley, although it are different in gene number by 1, it still shows a similar sequence and length. /note=Based on the given data on NCBI there is not known function on this gene. The hypothetical has a very high percent identity at 72.85% than the rest. Based on the HHpred there is a probability of of 81.5 that it is a biogenesis protein, but the phagesDB plast does not indicate a similar protein on the given phages with similar functions. CDS complement (58836 - 59126) /gene="97" /product="gp97" /function="hypothetical protein" /locus tag="fizzles_97" /note=Original Glimmer call @bp 59126 has strength 22.06; Genemark calls start at 59126 /note=SSC: 59126-58836 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_97 [Microbacterium phage Mashley]],,NCBI, q1:s1 93.75% 2.06605E-38 GAP: 39 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.711, -3.459652012164126, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_97 [Microbacterium phage Mashley]],,QED11913,76.3441,2.06605E-38 SIF-HHPRED: Jag_N ; Jag N-terminus,,,PF14804.6,18.75,49.1 SIF-Syn: The phamarator shows a close relationship between the gene 96 of Mashley and Fizzles base on length and position. /note=Based on HH pred, there`s a 77.92% probability that the protein is not characterize. NCBI blast shows 70.0% hypothetical protein (NKF). CDS complement (59166 - 59525) /gene="98" /product="gp98" /function="hypothetical protein" /locus tag="fizzles_98" /note=Original Glimmer call @bp 59525 has strength 16.98; Genemark calls start at 59525 /note=SSC: 59525-59166 CP: no SCS: both ST: NI BLAST-Start: [hypothetical protein PBI_HYPERION_98 [Microbacterium phage Hyperion]],,NCBI, q4:s5 95.7983% 9.4856E-42 GAP: -4 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.471, -3.750998330397795, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_98 [Microbacterium phage Hyperion]],,AWN03613,71.5517,9.4856E-42 SIF-HHPRED: SIF-Syn: /note=Could be a tail assembly chaperone because it contains a 100% frequency CDS complement (59522 - 59821) /gene="99" /product="gp99" /function="hypothetical protein" /locus tag="fizzles_99" /note=Original Glimmer call @bp 59821 has strength 18.91; Genemark calls start at 59821 /note=SSC: 59821-59522 CP: yes SCS: both ST: SS BLAST-Start: [unnamed protein product [Protopolystoma xenopodis]],,NCBI, q27:s311 39.3939% 9.12405 GAP: 49 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.471, -3.609999816735608, yes F: hypothetical protein SIF-BLAST: ,,[unnamed protein product [Protopolystoma xenopodis]],,VEL07986,5.11111,9.12405 SIF-HHPRED: conserved hypothetical protein; alpha-beta, dimeric alpha-beta barrels, Structural; 2.0A {Agrobacterium tumefaciens str.} SCOP: d.58.4.16,,,2FIU_B,23.2323,44.6 SIF-Syn: There are no similar genes. /note=There was no matches on NCBI and the HHpred matches were of very low percent. CDS complement (59871 - 60317) /gene="100" /product="gp100" /function="hypothetical protein" /locus tag="fizzles_100" /note=Original Glimmer call @bp 60317 has strength 11.91; Genemark calls start at 60317 /note=SSC: 60317-59871 CP: no SCS: both ST: NI BLAST-Start: GAP: 46 bp gap LO: no RBS: Kibler 6, Karlin Medium, 2.711, -3.7606820078281067, yes F: hypothetical protein SIF-BLAST: SIF-HHPRED: SIF-Syn: All data seems to point to something different from each other. Blastp does not show any similarities. HHpred has a high probability of being a lipid binding protein. When compared to similar phages, they do not show a function. /note=Blastp found no significant similarities. Phamerator does not show useful relating to fizzle`s gene 100 function. HHpred shows 96% probability of being a lipid binding protein. Has a 25% frequency of a rect-like protein. CDS complement (60364 - 60453) /gene="101" /product="gp101" /function="hypothetical protein" /locus tag="fizzles_101" /note=Genemark calls start at 60453 /note=SSC: 60453-60364 CP: no SCS: genemark ST: SS BLAST-Start: [hypothetical protein PBI_HYPERION_100 [Microbacterium phage Hyperion] ],,NCBI, q1:s1 100.0% 2.23429E-8 GAP: 43 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.52, -4.16062280240617, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_HYPERION_100 [Microbacterium phage Hyperion] ],,AWN03615,89.6552,2.23429E-8 SIF-HHPRED: GLUTAMINASE-ASPARAGINASE; BACTERIAL AMIDOHYDROLASE; HET: SO4; 1.7A {Pseudomonas sp. 7A} SCOP: c.88.1.1,,,4PGA_A,51.7241,57.4 SIF-Syn: This gene has an unknown function, because it is does not have a strong probability on HHpred or Blastp indicating it`s similarity to one particular protein. /note=HHpred gives a 51% probability of it being a Phosphoribosyl-AMP cyclohydrolase though the NCBI blast and phagesdb blast show that it is an unknown function. CDS complement (60497 - 60742) /gene="102" /product="gp102" /function="hypothetical protein" /locus tag="fizzles_102" /note=Original Glimmer call @bp 60742 has strength 5.97; Genemark calls start at 60802 /note=SSC: 60742-60497 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_102 [Microbacterium phage Mashley]],,NCBI, q1:s22 97.5309% 2.00567E-25 GAP: 56 bp gap LO: no RBS: Kibler 6, Karlin Medium, 1.442, -5.825397763003754, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_102 [Microbacterium phage Mashley]],,QED11918,56.4356,2.00567E-25 SIF-HHPRED: Tctex-1 ; Tctex-1 family,,,PF03645.13,13.5802,62.7 SIF-Syn: No conclusive evidence on Phamerator, NCBI, or HHpred. CDS complement (60799 - 61005) /gene="103" /product="gp103" /function="hypothetical protein" /locus tag="fizzles_103" /note=Original Glimmer call @bp 61005 has strength 7.77; Genemark calls start at 61005 /note=SSC: 61005-60799 CP: yes SCS: both ST: SS BLAST-Start: [hypothetical protein SEA_MASHLEY_103 [Microbacterium phage Mashley]],,NCBI, q1:s1 95.5882% 1.17648E-15 GAP: 186 bp gap LO: yes RBS: Kibler 6, Karlin Medium, 2.962, -2.723328252647382, yes F: hypothetical protein SIF-BLAST: ,,[hypothetical protein SEA_MASHLEY_103 [Microbacterium phage Mashley]],,QED11919,76.0563,1.17648E-15 SIF-HHPRED: Tetracenomycin polyketide synthesis protein tcmI; dimeric ??? ferredoxin-like fold tetracenomycin; 1.9A {Streptomyces glaucescens} SCOP: d.58.4.8,,,1TUW_A,26.4706,70.6 SIF-Syn: This gene is has a function of unknown because in blast it has a probability of hypothetical protein but in HHpred it contains another protein. CDS complement (61192 - 61995) /gene="104" /product="gp104" /function="hypothetical protein" /locus tag="fizzles_104" /note=Original Glimmer call @bp 61995 has strength 11.18; Genemark calls start at 61902 /note=SSC: 61995-61192 CP: yes SCS: both-gl ST: SS BLAST-Start: [hypothetical protein PBI_SQUASH_108 [Microbacterium phage Squash]],,NCBI, q38:s6 86.1423% 2.40591E-75 GAP: 0 bp gap LO: no RBS: Kibler 6, Karlin Medium, 0.905, -7.539442206964485, no F: hypothetical protein SIF-BLAST: ,,[hypothetical protein PBI_SQUASH_108 [Microbacterium phage Squash]],,AWN04726,71.8615,2.40591E-75 SIF-HHPRED: Hypothetical protein Rv1264/MT1302 (E.C.4.6.1.1); Alpha-helical, Regulatory Domain of Adenylyl; HET: OLA; 2.28A {Mycobacterium tuberculosis},,,2EV4_A,10.8614,40.9 SIF-Syn: This protein has no known function and is therefore classified as NKF. /note=Phagesdb lists this protein as a tape measure protein. However, comparisons through NCBI and HHpred prove that this gene has an unknown function.