{
  "license": "https://dustinedwards.info/terms",
  "count": 2,
  "protocols": [
    {
      "id": "phage-dna-extraction",
      "title": "Phage DNA Extraction Protocol",
      "url": "https://dustinedwards.info/research/protocols/phage-dna-extraction",
      "kind": "Protocol",
      "methods": [
        "Nucleic acid extraction"
      ],
      "organisms": [],
      "targets": [],
      "courses": [
        "Phage Discovery Program",
        "Virus Isolation Course"
      ],
      "steps": 22,
      "time": "",
      "version": "",
      "updated": "2026-09-30",
      "description": "Phage DNA extraction from a high-titer lysate by zinc chloride (ZnCl2) precipitation and TES, with no kit or column, plus DNA quantity and quality checks."
    },
    {
      "id": "phage-isolation",
      "title": "Phage Isolation and Purification Protocol",
      "url": "https://dustinedwards.info/research/protocols/phage-isolation",
      "kind": "Protocol",
      "methods": [
        "Plating and titering",
        "Culture"
      ],
      "organisms": [
        "Mycobacterium smegmatis",
        "Microbacterium foliorum"
      ],
      "targets": [],
      "courses": [
        "Phage Discovery Program",
        "Virus Isolation Course"
      ],
      "steps": 7,
      "time": "",
      "version": "",
      "updated": "2026-09-30",
      "description": "Tarleton SEA-PHAGES lab variants of the Phage Discovery Guide: direct vs enriched isolation, purification, spot titer, webbed plates, high titer lysate."
    }
  ]
}