{
  "license": "https://dustinedwards.info/terms",
  "count": 3,
  "protocols": [
    {
      "id": "coi-primers",
      "title": "COI primers: LCO1490 and HCO2198",
      "url": "https://dustinedwards.info/research/protocols/coi-primers",
      "kind": "Protocol",
      "methods": [
        "PCR"
      ],
      "organisms": [],
      "targets": [
        "COI (cytochrome c oxidase subunit I)"
      ],
      "courses": [],
      "steps": 2,
      "time": "",
      "version": "",
      "updated": "2026-09-30",
      "description": "LCO1490 and HCO2198 (Folmer) primer sequences for COI barcoding PCR of invertebrate mitochondrial DNA, with the cycling table, materials and product size."
    },
    {
      "id": "pan-avian-gapdh",
      "title": "Pan-avian GAPDH PCR",
      "url": "https://dustinedwards.info/research/protocols/pan-avian-gapdh",
      "kind": "Protocol",
      "methods": [
        "PCR"
      ],
      "organisms": [
        "Birds"
      ],
      "targets": [
        "GAPDH"
      ],
      "courses": [],
      "steps": 3,
      "time": "",
      "version": "",
      "updated": "2026-09-30",
      "description": "Pan-avian GAPDH primer sequences and PCR conditions, used as a control that a bird DNA extraction holds amplifiable avian DNA."
    },
    {
      "id": "rev-lpdv-primers",
      "title": "REV and LPDV PCR primers",
      "url": "https://dustinedwards.info/research/protocols/rev-lpdv-primers",
      "kind": "Protocol",
      "methods": [
        "PCR"
      ],
      "organisms": [
        "Birds"
      ],
      "targets": [
        "REV 3′ LTR",
        "REV pol",
        "LPDV p31/CA"
      ],
      "courses": [],
      "steps": 3,
      "time": "",
      "version": "",
      "updated": "2026-09-30",
      "description": "PCR primers for reticuloendotheliosis virus (REV) LTR and pol and for LPDV p31/CA: sequences, reaction mix, cycling tables and product sizes."
    }
  ]
}