COI primers: LCO1490 and HCO2198

Version not yet assigned, updated 2026-09-30. https://dustinedwards.info/research/protocols/coi-primers

Updated
2026-09-30

Reagents

ReagentAmountNotes
Promega GoTaq® Flexi DNA polymerasePromega GoTaq® Flexi DNA polymerase

Equipment

NEB 100 bp ladder; 1% agarose gel in TBE

Reaction mix

  1. Use your polymerase's recommended reaction mix. This page does not record the mix the lab used.

PCR conditions

  1. Run the product on the 1% agarose gel in TBE beside the 100 bp ladder and look for the band at 708 bp.

    Expected:

    The product at 708 bp, next to the 100 bp ladder, in the positive (mtDNA) lane, with no band in the negative lane.