REV pol (protease and reverse transcriptase) forward
- Sequence (5′ to 3′)
CAAATAATAGATTTTCTAGTAGATACGGGA- Reverse complement (5′ to 3′)
TCCCGTATCTACTAGAAAATCTATTATTTG- Direction
- forward
- Target
- REV pol
- Set
- PCR REV pol (protease and reverse transcriptase)
- Pairs with
- REV pol (protease and reverse transcriptase) reverse
- Product
- 574 bp
- Length
- 30 nt
- GC content
- 30%
- Melting temperature estimate (Tm)
- 54.9 °C
- Annealing temperature
- Depends on the polymerase: NEB Tm Calculator
- Source
- Stewart et al. 2019, J Wildl Dis 55(3) (on this site)
- Used in
The melting temperature (Tm) is an estimate for comparing primers with each other, computed from the sequence with the SantaLucia (1998) unified nearest-neighbour model (SantaLucia J Jr (1998), Proc Natl Acad Sci USA 95:1460-1465) at 50 mM monovalent cation and 0.5 µM primer. These are standard reporting conditions, not those of any one reaction buffer. A sequence with an ambiguity code has no single Tm and shows none. The Tm estimate is not an annealing temperature. For the annealing temperature of a particular polymerase, use the NEB Tm Calculator.