Pan-avian GAPDH reverse
- Sequence (5′ to 3′)
GGCAGCACCTCTGCCATC- Reverse complement (5′ to 3′)
GATGGCAGAGGTGCTGCC- Direction
- reverse
- Target
- GAPDH
- Set
- Pan-avian GAPDH PCR
- Pairs with
- Pan-avian GAPDH forward
- Product
- 534 bp
- Length
- 18 nt
- GC content
- 66.7%
- Melting temperature estimate (Tm)
- 60.8 °C
- Annealing temperature
- Depends on the polymerase: NEB Tm Calculator
- Source
- Olias et al. 2014
- Used in
The melting temperature (Tm) is an estimate for comparing primers with each other, computed from the sequence with the SantaLucia (1998) unified nearest-neighbour model (SantaLucia J Jr (1998), Proc Natl Acad Sci USA 95:1460-1465) at 50 mM monovalent cation and 0.5 µM primer. These are standard reporting conditions, not those of any one reaction buffer. A sequence with an ambiguity code has no single Tm and shows none. The Tm estimate is not an annealing temperature. For the annealing temperature of a particular polymerase, use the NEB Tm Calculator.