LCO1490
- Sequence (5′ to 3′)
GGTCAACAAATCATAAAGATATTGG- Reverse complement (5′ to 3′)
CCAATATCTTTATGATTTGTTGACC- Direction
- forward
- Target
- COI (cytochrome c oxidase subunit I)
- Set
- COI primers: LCO1490 and HCO2198
- Pairs with
- HCO2198
- Product
- about 710 bp
- Length
- 25 nt
- GC content
- 32%
- Melting temperature estimate (Tm)
- 53 °C
- Annealing temperature
- Depends on the polymerase: NEB Tm Calculator
- Source
- Folmer et al. 1994, Molecular Marine Biology and Biotechnology 3:294-299
- Used in
The melting temperature (Tm) is an estimate for comparing primers with each other, computed from the sequence with the SantaLucia (1998) unified nearest-neighbour model (SantaLucia J Jr (1998), Proc Natl Acad Sci USA 95:1460-1465) at 50 mM monovalent cation and 0.5 µM primer. These are standard reporting conditions, not those of any one reaction buffer. A sequence with an ambiguity code has no single Tm and shows none. The Tm estimate is not an annealing temperature. For the annealing temperature of a particular polymerase, use the NEB Tm Calculator.